Dopamine Induces Functional Extracellular Traps in Microglia

Simple item page
dc.contributor.advisorJha, Sushmita
dc.creator.researcherAgrawal, Ishan
dc.date.accessioned2023-12-06T10:42:35Z
dc.date.available2023-12-06T10:42:35Z
dc.date.awarded2021-12
dc.date.issued2021-12
dc.date.registered2015-07
dc.description.abstractExtracellular traps (ETs) are used by innate immune cells to trap pathogens. ETs kill pathogens and delay their dissemination giving time to other immune cells to come into action. Poor regulation of these traps may also lead to sterile inflammation and autoimmunity. We have investigated the role of dopamine (DA) in the formation of extracellular traps (ETs) in microglia. Microglia are the primary immune cells of central nervous system. We developed a robust and cost effective protocol for isolating primary adult human microglia. We report that DA can induce ETs in BV2 microglia cell line and primary adult human microglia. We investigated the mechanism of trap formation and found that microglia ETs are formed independent of cell death, reactive oxygen species and actin polymerization. DA induced traps were also functional as they trapped FITC tagged Escherichia coli. We also found the presence of microglia ETs in glioblastoma multiforme (GBM) tissues. We developed an unbiased algorithm for quantification of microglia ETs in GBM. These findings underline an important role of DA in regulation of sterile neuro-inflammation.en_US
dc.description.notecol. ill.; including bibliographyen_US
dc.description.statementofresponsibilityby Ishan Agrawalen_US
dc.format.accompanyingmaterialCDen_US
dc.format.extentxii, 69p.en_US
dc.identifier.accessionTP00112
dc.identifier.citationAgrawal, Ishan. (2021). Dopamine Induces Functional Extracellular Traps in Microglia (Doctor's thesis). Indian Institute of Technology Jodhpur, Jodhpur.en_US
dc.identifier.urihttps://ir.iitj.ac.in/handle/123456789/122
dc.language.isoen
dc.publisherIndian Institute of Technology Jodhpur
dc.publisher.departmentBioscience and Bioengineeringen_US
dc.publisher.placeJodhpur
dc.rights.holderIIT Jodhpur
dc.rights.licenseCC-BY-NC-SA
dc.subject.ddcBioscience & Bioengineeringen_US
dc.subject.ddcBiologyen_US
dc.subject.ddcExtracellular Trapsen_US
dc.subject.ddcMicroglia cellsen_US
dc.subject.ddcBiologyen_US
dc.titleDopamine Induces Functional Extracellular Traps in Microgliaen_US
dc.typeThesis
Files
Original bundle
Now showing 1 - 5 of 10
Thumbnail Image
Name:
01_title.pdf
Size:
377.95 KB
Format:
Adobe Portable Document Format
Download
Thumbnail Image
Name:
02_prelim pages.pdf
Size:
564.44 KB
Format:
Adobe Portable Document Format
Download
Thumbnail Image
Name:
03_table of contents.pdf
Size:
390.39 KB
Format:
Adobe Portable Document Format
Download
Thumbnail Image
Name:
04_abstract.pdf
Size:
344 KB
Format:
Adobe Portable Document Format
Download
Thumbnail Image
Name:
05_chapter 1.pdf
Size:
517.73 KB
Format:
Adobe Portable Document Format
Download
Collections
Doctoral Theses