A Solution state NMR Account of Organophosphate Pesticides - Protien Interaction.

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dc.contributor.advisorPal, Samanwita
dc.creator.researcherVandana
dc.date.accessioned2023-12-06T10:42:08Z
dc.date.available2023-12-06T10:42:08Z
dc.date.awarded2020-11
dc.date.issued2020-01
dc.date.registered2014-07
dc.description.abstractAnalysis and quantification of organophosphate pesticides (OP)-protein interaction draw major attention in the field of agrochemicals. Such molecular interactions have a significant role to play in the agro-ecosystem by affecting the non-target organisms. The current Thesis aims to investigate OP-protein interactions using solution-state 1D 1H NMR spectroscopy as a major technique complemented by fluorescence quenching studies, molecular docking, and Isothermal Titration Calorimetry (ITC), whenever required. The first part of the Thesis considers the interaction of OP and its metabolites with model protein Bovine Serum Albumin (BSA) employing 1H Saturation Transfer Difference (STD) NMR and selective 1H spin-lattice relaxation rate measurements methods. The analysis of experimental NMR data supported by molecular docking and ITC clearly indicated structure dependence of such molecular interaction, especially in the case of halogenated ligands. The highest binding affinity was exhibited by halogen-containing OP that pointed out the involvement of halogen bonding. Moreover, a pseudo-esterase activity was observed for BSA in the case of OP oxons.In the second part of the Thesis, the interaction of OP and their metabolites with gut enzyme trypsin in terms of the binding strength. The experimental data enabled the extraction of quantitative parameters of such interactions in terms of binding strength, thermodynamic parameters, and their binding modes. Also, the alteration in trypsin activity was monitored in the presence of OP. In a nutshell, this Thesis extends the applicability of in-vitro ligand-based solution-state 1H NMR methods with potential widespread application to characterize and understand the OP-protein interaction. The robustness emphasizes the conventional approach and future advantage of NMR application with computational methods, tailored to specific protein classes.en_US
dc.description.notecol. ill.; including bibliographyen_US
dc.description.statementofresponsibilityby Vandanaen_US
dc.format.accompanyingmaterialCDen_US
dc.format.extentxviii, 117p.en_US
dc.identifier.accessionTP00072
dc.identifier.citationVandana. (2020). A Solution state NMR Account of Organophosphate Pesticides - Protein Interaction (Doctor's thesis). Indian Institute of Technology Jodhpur, Jodhpur.en_US
dc.identifier.urihttps://ir.iitj.ac.in/handle/123456789/82
dc.language.isoen
dc.publisherIndian Institute of Technology Jodhpur
dc.publisher.departmentChemistryen_US
dc.publisher.placeJodhpur
dc.rights.holderIIT Jodhpur
dc.rights.licenseCC-BY-NC-SA
dc.subject.ddcChemistryen_US
dc.subject.ddcNMR Accounten_US
dc.subject.ddcOrganophosphate Pesticides - Protien Interactionen_US
dc.titleA Solution state NMR Account of Organophosphate Pesticides - Protien Interaction.en_US
dc.typeThesis
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